EVALUATION OF THE BIOTECHNOLOGICAL POTENTIAL OF ANTHOCYANS OF GRAPE RAW MATERIAL OF THE ODESSKIY CHORNY VARIETY
DOI:
https://doi.org/10.32782/tnv-tech.2024.6.25Keywords:
anthocyanin extracts, grape pomace, Odesa Black, natural dyes, biologically active compoundsAbstract
One of the actual problems is ensuring the safety and functionality of food and cosmetic products. One of the promising ways to solve it is the development and use of safe natural food dyes. These compounds, in addition to giving products color, are sources of biologically active substances, which makes it possible to form functional properties of final products. The purpose of this work is to assess the biotechnological potential of anthocyanins extracted from the pomace of the Odesa Black grapes. The assessment of anthocyanin dyes was carried out in accordance with the requirements of DSTU-N CODEX STAN 192:2014. To identify anthocyanins, the IR spectroscopy method was used using an IRTracer-100 spectrophotometer (Shimadzu, Japan). The extract was subjected to chromatographic separation using the Agilent 1200 Series HPLC–DAD system. Identification of anthocyanin extract components was performed using Agilent 6500 Series Accurate-Mass Quadrupole Time-of- Flight (Q-TOF) LC/MS QLC/TOF MS. The antiradical activity of anthocyanins and anthocyanin complexes with anionic polysaccharides was studied using the DPPH (2,2-diphenyl-1- picrylhydrazyl) method. The dependence of the anthocyanin content in the solution on the extraction time was studied using different extraction methods. The properties of anthocyanin dye obtained from grape pomace were studied. A clear mathematical dependence of the anthocyanin content in the solution on the extraction time was established using different extraction methods. It was determined that anthocyanin extracts from the pomace of the Odesa Black grapes exhibit antiradical activity. Assessment of the biotechnological potential of anthocyanins isolated from the pomace of the Odesa Black grapes showed that anthocyanin extracts can be used as dyes and sources of biologically active substances in the production of food products and cosmetics.
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